• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Domestic equipment for cultivation of cyanobacteria of the genera spirulina and arthrospira. Equipment comprising one or several containers dedicated to cultivation, of various shapes, provided with an electrical connection box having different heights and placed adjacent in a descending stepped manner, leaving all the containers joined at their lower part by means of a hollow base (10) in connection with a hollow rear wall (4) and with an electrical connection box (5), where the equipment also comprises: - a system for recirculating the culture or culture medium, - an aeration system for agitation of the crop, - a lighting system, - a heating system and - means for harvesting spirulina. You can count on: a washing system, a stabilization system and a grinding system. It allows the obtaining of spirulina and similar microorganisms domestically, for individual or family consumption, in a simple and effective way. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2616498A1
    申请号:ES201531792
    申请日:2015-12-10
    公开日:2017-06-13
    发明作者:María SEGURA FORNIELLS；Federico Guillermo WITT SOUSA
    申请人:Algaenergy S A；ALGAENERGY SA；
    IPC主号:
    专利说明:

    OBJECT OF THE INVENTION It is the object of the present invention, as the title of the same establishes, a domestic equipment for the cultivation of cyanobacteria of the genera Spirulina and Arthrospira, preferably of the photosynthetic microorganism called spirulina -or spirulina-, which is also valid to grow other similar microorganisms.
    Spirulina, declared by UNESCO as "the food of the millennium" is, in effect, a dietary supplement of very high value. It is a cyanobacterium of the genera Arthrospira and Spirulina, with the species Arfhrospira plafensis and Arfhrospira maxima being the most commonly cultivated.
    It characterizes the present invention the constructive particularities and the elements that are part of the equipment, in such a way that, having all these elements integrated, the equipment allows to grow spirulina and / or similar microorganisms domestically, in any type of environment and No need to have biotechnological knowledge.
    Therefore, the present invention is within the scope of culture equipment for marine photosynthetic microorganisms or phytoplankton
    (microalgae Ycyanobacteria),toocalledphotobioreactorsor
    photoreactors biological,Sohowinsideof theambitfromsayings
    microorganisms
    BACKGROUND OF THE INVENTION Spirulina is a prokaryotic organism whose cell is composed of a central region, where the nucleic acid (a single molecule of DNA) is located, a peripheral region where the thylakoid membranes are found that
    they contain chlorophyll and where photosynthesis is performed, and various inclusions or
    cytoplasmic structures, such as granules of polyphosphate, glycogen and cinanophicin, carboxisomas or polyhedral bodies, where RBP-carboxylase-oxygenase (RuBisCO, the main enzyme responsible for the
    photosynthetic fixation of carbon dioxide) and 70S ribosomes. The celutar wall It contains peptidoglycan and the structure and composition characteristic of
    Gram negative bacteria. The cells are surrounded by a layer of
    mucilage and are arranged one behind the other in multicellular filaments called trichomes. Arfhrospira trichomes are shaped like a helix
    levógira, approximately 0.5 mm long and 50 ~ m wide.
    The microorganism in question is found ubiquitously in numerous alkaline and brackish swamps (sodium lakes), shallow, in latitudes of great solar radiation, where it appears as a dominant species of phytoplankton
    due to the extreme conditions that favor its development and proliferation, to a greater degree than its competitors.
    The use of spirulina for food is not something new, since there is evidence that the Aztecs consumed it from the Lake of
    Texcoco. Also, other cultures in the Lake Chad area, such as
    Kanenmbu, also included in their usual diet spirulina in the form of cookies. Mankind has known this cyanobacteria for centuries, although
    it is in the last half of the twentieth century when it begins to be used
    industrially. In fact, its industrial cultivation did not begin until 1962 in the African country Chad, where it is called dihé. It is an ideal crop for
    arid areas in which the salinity and causticity of water makes it not It is suitable for use in traditional agriculture.
    Currently, the main use of spirulina is in the food and human nutrition sector, and is usually consumed in raw, mixed with liquids or dehydrated, sprinkled in salads, pasta or rice, but also in different formats as capsules,
    tablets or tablets, in the latter case in pressed format. In fact, it is becoming easier to find it in a greater number of dietary stores and even in many supermarkets, in the area of dietary supplements. It is also used as a source of pigments, such as phycocyanin or xanthophylls, or polyunsaturated fatty acids. Due to the high protein content, the replacement of soy protein or other traditional protein sources by spirulina has also been studied in the animal feed sector, in fact, the nickname of spirulina is "single-cell protein" or "single cell protein. " Spirulina is also commonly used as a bottom fish feed and is suitable for direct application as a facial or body cosmetic, biofertilizer, substrate amendment or conditioner, or biostimulant of higher plants.
    So far, a simple, modular, automated and portable method of domestic cultivation of spirulina and other similar microorganisms is unknown, therefore being the subject of the present invention a domestic equipment for the cultivation of spirulina and similar microorganisms, which does not require its correct use of biotechnological knowledge, as described below and is reflected in its essentiality in the first claim.
    DESCRIPTION OF THE INVENTION The object of the present invention is a domestic equipment for the cultivation of cyanobacteria of the Spirulina and Arthrospira genera comprising one or more containers dedicated to the cultivation, constructed of transparent material that allows the passage of sunlight or artificial light, favoring the photosynthetic process, the equipment being provided with an electrical connection box containing all the electrical and electronic elements, including the pump, the light programmer and the thermostats of the heating system.
    The equipment also has auxiliary means to the container described for cultivation, such as:
    a system for recirculation of the crop and culture medium,aeration system for crop agitation, elimination ofoxygen and atmospheric carbon supply,
    5 a lighting system andA heating system.
    The team also has:
    10 the necessary means for harvesting spirulina, which in turn comprises a biomass concentration system through filtration. the system for recirculation of the crop and of the culture medium, which can be by its mechanical drive or by a system
    15 "airlift"; in the case of the latter, it is necessary to include an additional air injection in the conduit installed for this purpose.
    In a possible preferred embodiment, but not limitative, therefore other embodiments are also possible, as already noted, the container dedicated to the cultivation comprises a series of containers of preferably rectangular prismatic shape, with different heights and placed next to each other, in a descending stepped manner, so that the water or culture medium of a container overflows over the adjoining one and so on, leaving all the containers joined at their bottom 25 by means of a hollow and watertight base for passage of conduits in connection, with a hollow back wall attached to the container of greater height and in whose upper or lateral part, it has the electrical connection box that contains all the elements such as the pump, the light programmer and the thermostats. The container dedicated to the culture can also be spherical or
    30 tubular, or a combination of them.
    The aeration system for crop agitation, oxygen removal and atmospheric carbon supply, has a conduit that, starting from an air pump (blower or similar) housed in the electrical connection box, runs to the bottom of the container , allowing the passage of the air flow through aerators or diffusers, which favor the rise of air bubbles through the hollow rear vertical wall and the hollow base, presenting a series of derivations in each of the containers so that the air propelled from the pump be transmitted to the nozzles or aerators or diffusers preferably arranged in the lower part of each container, thus achieving aeration of the culture of each container. As mentioned above, it is also possible that all this process is carried out in a single culture container, be it rectangular, spherical or tubular, adapting the bubbling to its shape.
    The lighting system, essential to provide the actinic radiation necessary for photosynthesis, in case of unavailability or insufficient sunlight, includes a power supply, a programmer and a series of luminaires, either led, fluorescent or any other type Another light mode. The luminaires are arranged in a way that allows the crop to be illuminated in an optimized and homogeneous way in each of the containers - or of the single container -, while the programmer and the power supply are housed in the junction box.
    The heating system, whose function is to allow the culture to be maintained in an optimum temperature range to favor cell growth, can be, among other feasible procedures but without this implying a limitation or restriction that excludes any other effective embodiment , by a radiating wire, heating base, coil or submerged resistance
    or heating jacket, or any other form of heat generation, which may be housed in the hollow base of the container or containers
    of cultivation The heating system will also have a programmer and a power supply housed in the junction box.
    The filtration system comprises a nylon mesh or similar, whose sieve passage allows the passage of water and the culture medium but retains the biomass for further processing, and which is only available when harvesting.
    In addition, the equipment can have means for processing, such as:
    a washing system, which allows the washing of the biomass harvested by water, replacing the culture medium with clean water. a drying stabilization system: optional drying process of the biomass washed by hot air or another method, to obtain a final product for dehydrated consumption. The biomass could also be stabilized by freezing, in a domestic freezer (the latter, not integrated into the present invention). Grinding system: consisting of the milling of dry biomass (optional)
    The equipment can be complemented with a pH indicator, the measurements can even be carried out in an automated way, by means of measurement systems housed in the electrical connection box. Similarly, the equipment can be complemented with a temperature indicator.
    Likewise, the equipment can be complemented with a spectrophotometric detector, similar to those incorporated in the classic biochemical component separation equipment, with a fixed wavelength (750 nm), indicating the moment at which there is a suitable cell density for Make the harvest. Counting even with an acoustic and / or luminous warning.
    The operation of the equipment is complemented by the following consumables:
    o Capsules or concentrated inoculum containers
    o Capsules or concentrated containers and / or sachets with the culture medium ingredients (initial)
    5 These two products are necessary to inoculate in the water the "strain" of the microalgae organism.
    In addition, the equipment must be complemented with other consumables.
    Capsules or concentrated containers and / or envelopes with the ingredients of culture medium (maintenance)
    10 Water adequacy capsules or containers, since, periodically, and once the biomass has been harvested, the water in the tanks must be "cleaned". In fact, periodically, with harvesting it is advisable to remove a piece of water from the container and refill it with treated or suitable water.
    15 Likewise, periodically and as recommended in the instructions for use, parts of the equipment that are in contact with the culture medium (containers, filters, etc.) should be cleaned. To this end, the equipment is designed in a way that allows easy decoupling of said parts.
    20 for best usability.
    The equipment is also designed in such a way that it allows to easily increase its productive capacity, by means of a simple interconnection system of one or several containers or cultivation areas, which can be
    25 conform as optional elements and accessories of the main equipment.
    Thanks to its peculiarities, the described equipment allows to produce and obtain spirulina biomass and similar microorganisms, in a domestic and small-scale way, in a simple and efficient way, suitable for direct consumption, both human and animal, as well as its direct application as a product
    facial or body cosmetic, as a biofertilizer or as a biostimulant forupper floors.
    Unless otherwise indicated, all technical and scientific elementsused herein have the meaning that usuallyIt is understood by the person skilled in the art to which this invention belongs. Inthe practice of the present invention procedures andmaterials similar or equivalent to those described herein.
    Throughout the description and the claims the word "comprises" andits variants are not intended to exclude other technical characteristics, additives,components or steps. For experts in the field, other objects, advantagesand features of the invention will be derived in part from the description andin part of the practice of the invention.
    EXPLANATION OF THE FIGURESTo complement the description that is being made and in order tohelp a better understanding of the features of the invention, ofaccording to a preferred example of practical realization thereof,accompanies, as an integral part of this description, a set of drawings inwhere, for illustrative and non-limiting purposes, the following has been represented:
    In Figure 1, we can see a schematic representation of thedomestic equipment for the cultivation of spirulina and the like.
    PREFERRED EMBODIMENT OF THE INVENTION.In view of the figures, an embodiment is described below.preferred and non-limiting of the proposed invention.
    In Figure 1 we can see that the equipment comprises, in the preferred embodiment shown, but not limited to, three containers (1), (2) and (3) that have a general prismatic shape, open superiorly and arranged contiguously forming a staggered configuration
    descending from the first container (1) to the third container (3),
    the containers being joined at the bottom by a hollow base (10) connected to the hollow vertical wall (4).
    The team includes:
    a system for recirculating the crop and the culture medium, an aeration system (6) for crop agitation, elimination of
    10 oxygen and atmospheric carbon supply, a heating system, a lighting system. In the embodiment shown, the recirculation system comprises a
    15 pump (5) that pumps the water or culture medium from the last container, in this case the third container (3) towards the top, by means of a
    conduit (11) that runs through the hollow base (10) and then through a coil (12) that runs through the hollow vertical part (4) to the junction box (9), where again the water or culture medium falls towards him
    20 first container (1). A glass wall detector and light sensor (750nm) could be housed in the hollow base or in the junction box.
    The aeration system (6) comprises a conduit (13) which, starting from a pump (not shown), housed in the upper case or housing
    25 electrical connection (9), runs through the hollow rear vertical wall (4) and the hollow base (10), presenting a series of branches in each of the containers so that the air driven from the pump is transmitted to the nozzles or aerators (14) preferably arranged in the part
    bottom of each container, thus achieving the aeration of water from each container.
    The lighting system comprises, in the embodiment shown, a series of
    LED strips (7) that illuminate the inside of each container.
    The whole set is covered by a cover (15) that covers all
    5 containers, avoiding spaces in contact with the outside.
    In order to harvest the spirulina produced, the equipment has a removable filtration system (8), which is disposed in the outlet (16) of the recirculation duct, so that the spirulina-bearing culture medium or similar it is passed through a mesh or sieve, of an equal mesh opening
    less than 50 micrometers, where spirulina is retained when it has reached a certain degree of densification.
    Describes sufficiently the nature of the present invention, as well as the
    15 way of putting it into practice, it is noted that, within its essentiality, it may be implemented in other embodiments that differ in detail from that indicated by way of example, and which will also achieve the protection sought , provided that it does not alter, change or modify its fundamental principle.
    权利要求:
    Claims (10)
    [1]
    1_-Domestic equipment for the cultivation of cyanobacteria of the Spirulina and Arthrospira genera, as well as other similar photosynthetic microorganisms characterized by comprising:
    one or more containers dedicated to cultivation, built in materialtransparent to allow the passage of sunlight or artificial light,thus favoring the photosynthetic process. Said container, of capacityvariable and different possible shapes, including rectangular ones,spherical and tubular,an electrical connection box that will contain all the elementsinherent to its correct operation, in particular, the pump, thelight programmer and thermostats,
    a system for recirculation of the crop and culture medium,an aeration system for crop agitation,a lighting systema heating system andmeans for harvesting spirulina, which in turn includesa filtration system and optionally drying.
    [2]
    2.-Domestic equipment for the cultivation of cyanobacteria of the genera Spirulina and Arthrospira, according to claim 1, characterized in that the container dedicated to the cultivation comprises a container or a series of them, of rectangular prismatic shape, open superiorly, and with different heights and placed next to each other in a descending staggered manner so that the water
    or culture medium of an overflowing container on the adjoining one and so on, all the containers being joined in its lower part by means of a hollow base for passage of conduits in connection with a rear hollow wall also attached to the container of greater height and in whose part Top or side features the electrical connection box.
    [3]
    3. - Domestic equipment for the cultivation of cyanobacteria of the Spirulina and Arthrospira genera, according to claim 1, characterized in that the system for recirculation of the culture and culture medium comprises a pump (5) that pumps the water or culture medium towards the upper part of the equipment through a duct (11) and then through a coil (12) to the junction box (9) where again the water or culture medium falls to the first container (1).
    [4]
    4.-Domestic equipment for the cultivation of cyanobacteria of the genera Spirulina and Arthrospira, according to any of the preceding claims, characterized in that the system for recirculation of the crop and culture medium is carried out by means of a mechanical drive system or by a uairlift system ", counting in this case with an additional aerator.
    [5]
    5.-Domestic equipment for cultivation of cyanobacteria of the Spirulina and Arthrospira genera, according to any of the preceding claims, characterized in that the aeration system for agitation of the crop comprises a conduit (13) that, starting from a pump housed in the box upper or electrical connection box (9), runs through the hollow rear vertical wall (4) and through the hollow base (10), presenting a series of branches in each of the containers, so that the air driven from the pump it is transmitted to the nozzles or aerators (14) arranged, preferably, in the lower part of each container.
    [6]
    6.-Domestic equipment for the cultivation of cyanobacteria of the Spirulina and Arthrospira genera, according to any of the preceding claims, characterized in that the lighting system comprises a power supply, a programmer and a series of luminaires, either of the LED type, or either fluorescent or any other type, where the luminaires are arranged in each of the containers, while the programmer and the power supply are housed in the junction box (9).
    [7]
    7.-Domestic equipment for the cultivation of cyanobacteria of the Spirulina and Arthrospira genera, according to any of the preceding claims, characterized in that the heating system is carried out by any of the following methods: radiant wire, resistors, heating base, coil
    or heating jacket; and it has a programmer and a power supply housed in the junction box.
    [8]
    8.-Domestic equipment for the cultivation of cyanobacteria of the genera Spirulina and Arthrospira, according to any of the preceding claims, characterized in that, if the heating method is by radiating wire, it is submerged in contact with the crop.
    [9]
    9.-Domestic equipment for the cultivation of cyanobacteria of the Spirulina and Arthrospira genera, according to any of the preceding claims, characterized in that the filtration system comprises a nylon mesh, of no more than 50 ~ m mesh opening, which allows the passage of water or culture medium but that retains the biomass for further processing.
    [10]
    10.-Domestic equipment for the cultivation of cyanobacteria of the genera Spirulina and Arthrospira, according to any of the preceding claims, characterized in that the equipment has means for processing, comprising:
    a washing system, which allows the washing of harvested biomass
    by water and facilitates the replacement of the culture medium with water
    clean
    a drying stabilization system for drying biomass
    washed, by hot air, to obtain a final product
    dehydrated that is suitable for consumption or application.
    Grinding system for milling dry biomass.
    11 _-Domestic equipment for the cultivation of cyanobacteria of the Spirulina and Arthrospira genera, according to any of the preceding claims, characterized in that the equipment has a pH indicator
    12_-Domestic equipment for the cultivation of cyanobacteria of the Spirulina genera and
    Arthrospira, according to any of the preceding claims, characterized in that the equipment has a temperature indicator and a spectrophotometric detector indicating the appropriate time to harvest according to the cell density.
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    同族专利:
    公开号 | 公开日
    ES2616498B1|2017-10-09|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US6156561A|1997-09-16|2000-12-05|Spirulina Biological Lab., Ltd.|System and method for culturing algae|
    US6698134B1|1998-11-16|2004-03-02|Succeed Hi-Tech Industrialization Scale-Up Assemblies Co., Ltd.|Method of cultivating fresh spirulina at home and device thereof|
    法律状态:
    2016-11-18| PC2A| Transfer of patent|Owner name: ALGAENERGY, S.A. Effective date: 20161114 |
    2017-10-09| FG2A| Definitive protection|Ref document number: 2616498 Country of ref document: ES Kind code of ref document: B1 Effective date: 20171009 |
    优先权:
    申请号 | 申请日 | 专利标题
    ES201531792A|ES2616498B1|2015-12-10|2015-12-10|DOMESTIC EQUIPMENT FOR CULTURAL CULTURE OF THE SPIRULINA AND ARTHROSPIRA GENDERS|ES201531792A| ES2616498B1|2015-12-10|2015-12-10|DOMESTIC EQUIPMENT FOR CULTURAL CULTURE OF THE SPIRULINA AND ARTHROSPIRA GENDERS|
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